Journal: Pathology and Oncology Research

Article Title: Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers

doi: 10.3389/pore.2021.625438

Figure Lengend Snippet: Regional difference in TRAF2 and TRAF3 frameshift mutation in colon cancers. Sanger DNA sequencing shows regional difference in TRAF2 c.26delC (A) and TRAF3 c.854dupA (B) (mutant (MT) and wild-type (WT)).

Article Snippet: For the expression, we analyzed both TRAF2 and TRAF3 protein expressions in the CCs by immunohistochemistry with anti-TRAF2 antibody (Atlas Antibodies, Stockholm, Sweden; dilution 1/50) and anti-TRAF3 antibody (LSBio, Seattle, WA, United States; dilution 1/200), respectively.

Techniques: Mutagenesis, DNA Sequencing

Journal: Pathology and Oncology Research

Article Title: Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers

doi: 10.3389/pore.2021.625438

Figure Lengend Snippet: TRAF2 and TRAF3 expressions in colon cancers. (A – C ) Normal colonic mucosal epithelial cells (N) show negative TRAF2 immunostaining. (B) : Colon cancer (T) and normal (N) cells show negative TRAF2 immunostaining. (C,D) : In colon cancers, the cancer cells (T) show positive TRAF2 immunostaining, which is compared to negative TRAF2 immunostaining in normal cells (N). (E) Normal colonic mucosal epithelial cells show weakly positive TRAF3 immunostaining. (F) Colon cancer (T) shows negative TRAF3 immunostaining while non-tumor mucosal cells are weakly positive (N). (G) Colon cancer cells show positive TRAF3 immunostaining. (H) Colon cancer cells show negative TRAF3.

Article Snippet: For the expression, we analyzed both TRAF2 and TRAF3 protein expressions in the CCs by immunohistochemistry with anti-TRAF2 antibody (Atlas Antibodies, Stockholm, Sweden; dilution 1/50) and anti-TRAF3 antibody (LSBio, Seattle, WA, United States; dilution 1/200), respectively.

Techniques: Immunostaining

Journal: Biochemistry and Biophysics Reports

Article Title: The miR-2110/ TRAF3 axis is associated with endothelial dysfunction and atherosclerosis in coronary heart disease

doi: 10.1016/j.bbrep.2026.102508

Figure Lengend Snippet: Validation of TRAF3 as a direct target of miR-2110 by RT-qPCR, luciferase reporter assay, and Western blot analysis ( A ) RT-qPCR analysis of TRAF3 mRNA levels in OE2110 and OENC cells, calculated using the 2 −ΔΔCt method, normalized to GAPDH, and expressed relative to the control group (set to 1). ( B ) TRAF3 mRNA levels in peripheral blood samples from healthy controls (n = 30) and CHD patients (n = 34), normalized to GAPDH. ( C ) Schematic representation of the predicted binding sites between miR-2110 and the wild-type (WT) 3′-UTR of TRAF3 , and the corresponding mutated (MT) sequence used for luciferase assays. ( D ) Dual-luciferase reporter assay showing decreased luciferase activity in cells co-transfected with miR-2110 mimics and TRAF3 -WT but not TRAF3 -MT. ( E, F ) Western blot analysis and quantification of TRAF3 protein expression in OE2110 and OENC, with GAPDH as internal reference. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ns: not significant. Panels A, D, and F: Data are presented as mean ± SD from three independent biological experiments (N = 3) using EA.hy926 cells. Panel E: Representative blots are shown. Statistical comparisons were performed using unpaired two-tailed Student's t-tests. Panel B (patient samples): Data were presented as median (interquartile range) and were analyzed using the Mann–Whitney U test.

Article Snippet: Endogenous peroxidase activity was quenched with 3% H 2 O 2 for 10 min at room temperature, followed by blocking with 5% normal goat serum (Solarbio, China, SL038) in PBS for 1 h. Sections were incubated overnight at 4 °C with rabbit anti-TRAF3 monoclonal antibody (1:200, HUABIO, China, PSH01-30), then probed with HRP-conjugated goat anti-rabbit IgG (1:500, Servicebio, China, GB23303) for 1 h at room temperature.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Luciferase, Reporter Assay, Western Blot, Control, Binding Assay, Sequencing, Activity Assay, Transfection, Expressing, Two Tailed Test, MANN-WHITNEY

Journal: Biochemistry and Biophysics Reports

Article Title: The miR-2110/ TRAF3 axis is associated with endothelial dysfunction and atherosclerosis in coronary heart disease

doi: 10.1016/j.bbrep.2026.102508

Figure Lengend Snippet: Increased TRAF3 expression in cardiac and aortic tissues of ApoE −/− mice fed a high-fat diet ( A ) Relative Traf3 mRNA expression in the aortic root and adjacent heart tissue quantified by RT-qPCR, calculated using the 2 −ΔΔCt method, normalized to GAPDH, and expressed relative to the control group (set to 1). ( B, C ) Western blot analysis and quantification of TRAF3 protein expression in heart tissues. ( D, E ) Western blot analysis and quantification of TRAF3 protein expression in aortic tissues. ( F ) Representative immunohistochemical staining of TRAF3 in aortic root sections from control and ApoE −/− HFD mice, showing predominant localization in the tunica intima, including the endothelial layer and subendothelial regions containing vascular smooth muscle cells and infiltrating leukocytes (200 × ). Red boxes highlight regions with enhanced staining in HFD mice. (G) Quantification of TRAF3-positive area in the tunica intima. ∗ P < 0.05. Data represent mean ± SD; n = 6 mice per group. Statistical comparisons were performed using unpaired two-tailed Student's t-tests.

Article Snippet: Endogenous peroxidase activity was quenched with 3% H 2 O 2 for 10 min at room temperature, followed by blocking with 5% normal goat serum (Solarbio, China, SL038) in PBS for 1 h. Sections were incubated overnight at 4 °C with rabbit anti-TRAF3 monoclonal antibody (1:200, HUABIO, China, PSH01-30), then probed with HRP-conjugated goat anti-rabbit IgG (1:500, Servicebio, China, GB23303) for 1 h at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Immunohistochemical staining, Staining, Two Tailed Test